Table of contents

Summary

GAF summary

Found 144168 annotations in 144184 lines.

GAF Validation Summary Errors are reported first.

For rule GO_AR:0000005 (http://www.geneontology.org/GO.annotation_qc.shtml#GO_AR:0000005) No ISS or ISS-related annotations to 'protein binding ; GO:0005515', there are 3 violations with type Error.

For rule GO_AR:0000014 (http://www.geneontology.org/GO.annotation_qc.shtml#GO_AR:0000014) Valid GO term ID, there are 31 violations with type Error.

For rule GO_AR:0000003 (http://www.geneontology.org/GO.annotation_qc.shtml#GO_AR:0000003) Annotations to 'binding ; GO:0005488' and 'protein binding ; GO:0005515' should be made with IPI and an interactor in the 'with' field, there are 3 violations with type Warning.

GAF Prediction Summary

Found 33 predictions, see prediction file for details.

Used Ontology Summary

go/extensions/go-gaf

Report

SUMMARY

STATISTICS

MESSAGES

ERROR

WARNING

OWLTools check

#Line number RuleID ViolationType Message Line
#------------
#------------
# Validation for #144168 annotations in 144184 lines
#------------
#Line number RuleID ViolationType Message Line
#------------
# GO_AR:0000005 No ISS or ISS-related annotations to 'protein binding ; GO:0005515' Error count: 3
128049 GO_AR:0000005 Error No ISS or ISS-related annotations to 'protein binding ; GO:0005515' UniProtKB K7GQZ2 TGFB1 GO:0005515 GO_REF:0000024 ISS UniProtKB:P01137 F Transforming growth factor beta-1 TGFB1 protein taxon:9823 20141202 AgBase
132277 GO_AR:0000005 Error No ISS or ISS-related annotations to 'protein binding ; GO:0005515' UniProtKB P07200 TGFB1 GO:0005515 GO_REF:0000024 ISS UniProtKB:P01137 F Transforming growth factor beta-1 TGFB1 protein taxon:9823 20141202 AgBase
137340 GO_AR:0000005 Error No ISS or ISS-related annotations to 'protein binding ; GO:0005515' UniProtKB Q28833 VWF GO:0005515 GO_REF:0000024 ISS UniProtKB:P04275 F von Willebrand factor VWF|F8VWF protein taxon:9823 20070328 UniProt
#------------
# GO_AR:0000014 Valid GO term ID Error count: 31
3876 GO_AR:0000014 Error The id 'GO:0001076' in the annotation is an obsolete class UniProtKB A0A286ZMU2 MYOCD GO:0001076 GO_REF:0000107 IEA UniProtKB:Q8VIM5|ensembl:ENSMUSP00000104335 F Myocardin MYOCD protein taxon:9823 20180714 Ensembl
4551 GO_AR:0000014 Error The id 'GO:0001076' in the annotation is an obsolete class UniProtKB A0A286ZNK4 YAP1 GO:0001076 GO_REF:0000107 IEA UniProtKB:P46938|ensembl:ENSMUSP00000069554 F Yes associated protein 1 YAP1 protein taxon:9823 20180714 Ensembl
14659 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB A0A287A1X6 NR1H4 GO:0001190 GO_REF:0000107 IEA UniProtKB:Q96RI1|ensembl:ENSP00000447149 F Bile acid receptor NR1H4 protein taxon:9823 20180714 Ensembl
22410 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB A0A287AD23 NKX2-5 GO:0001190 GO_REF:0000107 IEA UniProtKB:P42582|ensembl:ENSMUSP00000015723 F NK2 homeobox 5 NKX2-5 protein taxon:9823 20180714 Ensembl
27688 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB A0A287AJS5 MED12 GO:0001190 GO_REF:0000107 IEA UniProtKB:A2AGH6|ensembl:ENSMUSP00000085260 F Mediator complex subunit 12 MED12 protein taxon:9823 20180714 Ensembl
34963 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB A0A287AVH0 CREB1 GO:0001190 GO_REF:0000107 IEA UniProtKB:P16220|ensembl:ENSP00000387699 F cAMP responsive element binding protein 1 CREB1 protein taxon:9823 20180714 Ensembl
36497 GO_AR:0000014 Error The id 'GO:0001191' in the annotation is an obsolete class UniProtKB A0A287AXH9 TBXT GO:0001191 GO_REF:0000107 IEA UniProtKB:P20293|ensembl:ENSMUSP00000074236 F T-box transcription factor T TBXT protein taxon:9823 20180714 Ensembl
37121 GO_AR:0000014 Error The id 'GO:0000991' in the annotation is an obsolete class UniProtKB A0A287AYH6 SUPT6H GO:0000991 GO_REF:0000104 IEA UniRule:UR000638772 F Transcription elongation factor spt6 SUPT6H protein taxon:9823 20180714 UniProt
37418 GO_AR:0000014 Error The id 'GO:0001191' in the annotation is an obsolete class UniProtKB A0A287AYR6 BHLHE41 GO:0001191 GO_REF:0000107 IEA UniProtKB:Q99PV5|ensembl:ENSMUSP00000032386 F Basic helix-loop-helix family member e41 BHLHE41 protein taxon:9823 20180714 Ensembl
39505 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB A0A287B1L8 NOTCH1 GO:0001190 GO_REF:0000107 IEA UniProtKB:Q01705|ensembl:ENSMUSP00000028288 F Notch 1 NOTCH1 protein taxon:9823 20180714 Ensembl
42049 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB A0A287B547 ARNTL GO:0001190 GO_REF:0000107 IEA UniProtKB:Q9WTL8|ensembl:ENSMUSP00000147989 F Aryl hydrocarbon receptor nuclear translocator-like protein 1 ARNTL protein taxon:9823 20180714 Ensembl
42095 GO_AR:0000014 Error The id 'GO:0001191' in the annotation is an obsolete class UniProtKB A0A287B554 TBX6 GO:0001191 GO_REF:0000107 IEA UniProtKB:P70327|ensembl:ENSMUSP00000091579 F T-box 6 TBX6 protein taxon:9823 20180714 Ensembl
47359 GO_AR:0000014 Error The id 'GO:0000989' in the annotation is an obsolete class UniProtKB A0A287BCF4 MSX2 GO:0000989 GO_REF:0000107 IEA UniProtKB:Q03358|ensembl:ENSMUSP00000021922 F Msh homeobox 2 MSX2 protein taxon:9823 20180714 Ensembl
49095 GO_AR:0000014 Error The id 'GO:0001191' in the annotation is an obsolete class UniProtKB A0A287BEM0 BHLHE40 GO:0001191 GO_REF:0000107 IEA UniProtKB:O14503|ensembl:ENSP00000256495 F Class E basic helix-loop-helix protein 40 BHLHE40 protein taxon:9823 20180714 Ensembl
55774 GO_AR:0000014 Error The id 'GO:0001191' in the annotation is an obsolete class UniProtKB A0A287BPC2 SPEN GO:0001191 GO_REF:0000107 IEA UniProtKB:Q96T58|ensembl:ENSP00000364912 F Spen family transcriptional repressor SPEN protein taxon:9823 20180714 Ensembl
63201 GO_AR:0000014 Error The id 'GO:0001076' in the annotation is an obsolete class UniProtKB C4TJC6 Lhx2 GO:0001076 GO_REF:0000107 IEA UniProtKB:Q9Z0S2|ensembl:ENSMUSP00000000253 F LIM homeobox protein 2 Lhx2|LHX2 protein taxon:9823 20180714 Ensembl
67923 GO_AR:0000014 Error The id 'GO:0001076' in the annotation is an obsolete class UniProtKB F1RIQ5 MAMSTR GO:0001076 GO_REF:0000107 IEA UniProtKB:Q0ZCJ7|ensembl:ENSMUSP00000114686 F MEF2 activating motif and SAP domain containing transcriptional regulator MAMSTR protein taxon:9823 20180714 Ensembl
69156 GO_AR:0000014 Error The id 'GO:0001191' in the annotation is an obsolete class UniProtKB F1RK46 CREBBP GO:0001191 GO_REF:0000107 IEA UniProtKB:Q92793|ensembl:ENSP00000262367 F CREB binding protein CREBBP protein taxon:9823 20180714 Ensembl
74499 GO_AR:0000014 Error The id 'GO:0001076' in the annotation is an obsolete class UniProtKB F1RRM9 SRF GO:0001076 GO_REF:0000107 IEA UniProtKB:Q9JM73|ensembl:ENSMUSP00000015749 F Serum response factor SRF protein taxon:9823 20180714 Ensembl
75591 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB F1RSP3 FOXH1 GO:0001190 GO_REF:0000107 IEA UniProtKB:O88621|ensembl:ENSMUSP00000036591 F Forkhead box H1 FOXH1 protein taxon:9823 20180714 Ensembl
77913 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB F1RVI1 CLOCK GO:0001190 GO_REF:0000107 IEA UniProtKB:O08785|ensembl:ENSMUSP00000143939 F Clock circadian regulator CLOCK protein taxon:9823 20180714 Ensembl
78743 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB F1RWH5 NFE2L1 GO:0001190 GO_REF:0000107 IEA UniProtKB:Q61985|ensembl:ENSMUSP00000080467 F Nuclear factor, erythroid 2 like 1 NFE2L1 protein taxon:9823 20180714 Ensembl
96248 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB F1SGS5 LMO2 GO:0001190 GO_REF:0000107 IEA UniProtKB:P25791|ensembl:ENSP00000257818 F LIM domain only 2 LMO2 protein taxon:9823 20180714 Ensembl
100925 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB F1SM11 GBX2 GO:0001190 GO_REF:0000107 IEA UniProtKB:P48031|ensembl:ENSMUSP00000048508 F Gastrulation brain homeobox 2 GBX2 protein taxon:9823 20180714 Ensembl
101114 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB F1SM76 PPARA GO:0001190 GO_REF:0000107 IEA UniProtKB:P23204|ensembl:ENSMUSP00000105049 F Peroxisome proliferator-activated receptor alpha PPARA protein taxon:9823 20180714 Ensembl
109405 GO_AR:0000014 Error The id 'GO:0000989' in the annotation is an obsolete class UniProtKB F2Z5G6 LDB1 GO:0000989 GO_REF:0000107 IEA UniProtKB:P70662|ensembl:ENSMUSP00000139562 F LIM domain-binding protein 1 LDB1 protein taxon:9823 20180714 Ensembl
117103 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB I3LG84 WWP2 GO:0001190 GO_REF:0000107 IEA UniProtKB:Q9DBH0|ensembl:ENSMUSP00000132224 F E3 ubiquitin-protein ligase WWP2 protein taxon:9823 20180714 Ensembl
122691 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB I3LR22 MIXL1 GO:0001190 GO_REF:0000107 IEA UniProtKB:Q9WUI0|ensembl:ENSMUSP00000027778 F Mix paired-like homeobox MIXL1 protein taxon:9823 20180714 Ensembl
126257 GO_AR:0000014 Error The id 'GO:0001190' in the annotation is an obsolete class UniProtKB K7GM20 KLF4 GO:0001190 GO_REF:0000107 IEA UniProtKB:O43474|ensembl:ENSP00000363804 F Krueppel-like factor 4 KLF4 protein taxon:9823 20180714 Ensembl
129995 GO_AR:0000014 Error The id 'GO:0001191' in the annotation is an obsolete class UniProtKB M3V811 MINA GO:0001191 GO_REF:0000107 IEA UniProtKB:Q8CD15|ensembl:ENSMUSP00000125297 F MYC induced nuclear antigen tv2 MINA|RIOX2 protein taxon:9823 20180714 Ensembl
131485 GO_AR:0000014 Error The id 'GO:0001191' in the annotation is an obsolete class UniProtKB P00636 FBP1 GO:0001191 GO_REF:0000107 IEA UniProtKB:P09467|ensembl:ENSP00000408025 F Fructose-1,6-bisphosphatase 1 FBP1|FBP protein taxon:9823 20180714 Ensembl
#------------
# GO_AR:0000003 Annotations to 'binding ; GO:0005488' and 'protein binding ; GO:0005515' should be made with IPI and an interactor in the 'with' field Warning count: 3
128049 GO_AR:0000003 Warning Annotations to 'binding' (GO:0005488) and 'protein binding' (GO:0005515) should be made with an IPI evidence code and the interactor in the 'with' field UniProtKB K7GQZ2 TGFB1 GO:0005515 GO_REF:0000024 ISS UniProtKB:P01137 F Transforming growth factor beta-1 TGFB1 protein taxon:9823 20141202 AgBase
132277 GO_AR:0000003 Warning Annotations to 'binding' (GO:0005488) and 'protein binding' (GO:0005515) should be made with an IPI evidence code and the interactor in the 'with' field UniProtKB P07200 TGFB1 GO:0005515 GO_REF:0000024 ISS UniProtKB:P01137 F Transforming growth factor beta-1 TGFB1 protein taxon:9823 20141202 AgBase
137340 GO_AR:0000003 Warning Annotations to 'binding' (GO:0005488) and 'protein binding' (GO:0005515) should be made with an IPI evidence code and the interactor in the 'with' field UniProtKB Q28833 VWF GO:0005515 GO_REF:0000024 ISS UniProtKB:P04275 F von Willebrand factor VWF|F8VWF protein taxon:9823 20070328 UniProt
#------------

Predictions

GO:0120009 intermembrane lipid transfer AnnotationPropagation P GO:0120013 intermembrane lipid transfer activity ISS
GO:0055114 oxidation-reduction process AnnotationPropagation P GO:0097257 leukotriene B4 12-hydroxy dehydrogenase activity TAS
GO:0005739 mitochondrion AnnotationPropagation C GO:1901029 negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0090615 mitochondrial mRNA processing ISS
GO:0005623 cell AnnotationPropagation C GO:0035356 cellular triglyceride homeostasis ISS
GO:0005622 intracellular AnnotationPropagation C GO:0097193 intrinsic apoptotic signaling pathway ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0097345 mitochondrial outer membrane permeabilization ISS
GO:0005622 intracellular AnnotationPropagation C GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway ISS
GO:0071108 protein K48-linked deubiquitination AnnotationPropagation P GO:1990380 Lys48-specific deubiquitinase activity ISS
GO:0005622 intracellular AnnotationPropagation C GO:0072425 signal transduction involved in G2 DNA damage checkpoint ISS
GO:0005622 intracellular AnnotationPropagation C GO:0097193 intrinsic apoptotic signaling pathway ISS
GO:0005622 intracellular AnnotationPropagation C GO:0042147 retrograde transport, endosome to Golgi ISS
GO:0005623 cell AnnotationPropagation C GO:0042147 retrograde transport, endosome to Golgi ISS
GO:0005829 cytosol AnnotationPropagation C GO:0042147 retrograde transport, endosome to Golgi ISS
GO:0006508 proteolysis AnnotationPropagation P GO:0097199 cysteine-type endopeptidase activity involved in apoptotic signaling pathway ISS
GO:0006915 apoptotic process AnnotationPropagation P GO:0097199 cysteine-type endopeptidase activity involved in apoptotic signaling pathway ISS
GO:0097190 apoptotic signaling pathway AnnotationPropagation P GO:0097199 cysteine-type endopeptidase activity involved in apoptotic signaling pathway ISS
GO:0005623 cell AnnotationPropagation C GO:0051480 regulation of cytosolic calcium ion concentration ISS
GO:0010452 histone H3-K36 methylation AnnotationPropagation P GO:0046975 histone methyltransferase activity (H3-K36 specific) ISS
GO:0005622 intracellular AnnotationPropagation C GO:0071880 adenylate cyclase-activating adrenergic receptor signaling pathway ISS
GO:0006468 protein phosphorylation AnnotationPropagation P GO:0050254 rhodopsin kinase activity ISS
GO:0016311 dephosphorylation AnnotationPropagation P GO:0052834 inositol monophosphate phosphatase activity ISS
GO:0006468 protein phosphorylation AnnotationPropagation P GO:0070123 transforming growth factor beta receptor activity, type III ISS
GO:0007178 transmembrane receptor protein serine/threonine kinase signaling pathway AnnotationPropagation P GO:0070123 transforming growth factor beta receptor activity, type III ISS
GO:0006508 proteolysis AnnotationPropagation P GO:0070006 metalloaminopeptidase activity ISS
GO:0030974 thiamine pyrophosphate transmembrane transport AnnotationPropagation P GO:0090422 thiamine pyrophosphate transmembrane transporter activity ISS
GO:0035461 vitamin transmembrane transport AnnotationPropagation P GO:0090422 thiamine pyrophosphate transmembrane transporter activity ISS
GO:0005622 intracellular AnnotationPropagation C GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress ISS
GO:0005622 intracellular AnnotationPropagation C GO:0071880 adenylate cyclase-activating adrenergic receptor signaling pathway ISS
GO:0005623 cell AnnotationPropagation C GO:0051480 regulation of cytosolic calcium ion concentration ISS
GO:0006858 extracellular transport FoldBasedPredictor GO:0045056 transcytosis occurs_in GO:0034364 high-density lipoprotein particle occurs_in UBERON:0000201 endothelial blood brain barrier IMP
GO:0034364 high-density lipoprotein particle FoldBasedPredictor GO:0045056 transcytosis occurs_in GO:0034364 high-density lipoprotein particle occurs_in UBERON:0000201 endothelial blood brain barrier IMP
GO:0097223 sperm part FoldBasedPredictor GO:0045177 apical part of cell exists_during GO:0048240 sperm capacitation part_of CL:0000019 sperm IDA

Predictions (experimental)