Table of contents

Summary

GAF summary

Found 146646 annotations in 146662 lines.

GAF Validation Summary Errors are reported first.

For rule GO_AR:0000005 (http://www.geneontology.org/GO.annotation_qc.shtml#GO_AR:0000005) No ISS or ISS-related annotations to 'protein binding ; GO:0005515', there are 6 violations with type Error.

For rule GO_AR:0000003 (http://www.geneontology.org/GO.annotation_qc.shtml#GO_AR:0000003) Annotations to 'binding ; GO:0005488' and 'protein binding ; GO:0005515' should be made with IPI and an interactor in the 'with' field, there are 7 violations with type Warning.

GAF Prediction Summary

Found 150 predictions, see prediction file for details.

Used Ontology Summary

go/extensions/go-gaf

Report

SUMMARY

STATISTICS

MESSAGES

ERROR

OWLTools check

#Line number RuleID ViolationType Message Line
#------------
#------------
# Validation for #146646 annotations in 146662 lines
#------------
#Line number RuleID ViolationType Message Line
#------------
# GO_AR:0000005 No ISS or ISS-related annotations to 'protein binding ; GO:0005515' Error count: 6
39480 GO_AR:0000005 Error No ISS or ISS-related annotations to 'protein binding ; GO:0005515' UniProtKB E1BNK3 TGFB1 GO:0005515 GO_REF:0000024 ISS UniProtKB:P01137 F Transforming growth factor beta-1 TGFB1|TGFB1 protein taxon:9913 20141202 AgBase
100960 GO_AR:0000005 Error No ISS or ISS-related annotations to 'protein binding ; GO:0005515' UniProtKB P08169 IGF2R GO:0005515 GO_REF:0000024 ISS UniProtKB:P11717 F Cation-independent mannose-6-phosphate receptor IGF2R|IGF2R|M6P protein taxon:9913 20091210 UniProt
102764 GO_AR:0000005 Error No ISS or ISS-related annotations to 'protein binding ; GO:0005515' UniProtKB P18341 TGFB1 GO:0005515 GO_REF:0000024 ISS UniProtKB:P01137 F Transforming growth factor beta-1 TGFB1|TGFB1 protein taxon:9913 20141202 AgBase
109065 GO_AR:0000005 Error No ISS or ISS-related annotations to 'protein binding ; GO:0005515' UniProtKB P80012 VWF GO:0005515 GO_REF:0000024 ISS UniProtKB:P04275 F von Willebrand factor VWF|VWF|F8VWF protein taxon:9913 20070328 UniProt
131516 GO_AR:0000005 Error No ISS or ISS-related annotations to 'protein binding ; GO:0005515' UniProtKB Q3MHX6 OS9 GO:0005515 GO_REF:0000024 ISS UniProtKB:Q13438 F Protein OS-9 OS9|OS9 protein taxon:9913 20090907 UniProt
131958 GO_AR:0000005 Error No ISS or ISS-related annotations to 'protein binding ; GO:0005515' UniProtKB Q3SX24 FBXO6 GO:0005515 GO_REF:0000024 ISS UniProtKB:Q9QZN4 F F-box only protein 6 FBXO6|FBXO6|FBS2|FBX6 protein taxon:9913 20090903 UniProt
#------------
# GO_AR:0000003 Annotations to 'binding ; GO:0005488' and 'protein binding ; GO:0005515' should be made with IPI and an interactor in the 'with' field Warning count: 7
39480 GO_AR:0000003 Warning Annotations to 'binding' (GO:0005488) and 'protein binding' (GO:0005515) should be made with an IPI evidence code and the interactor in the 'with' field UniProtKB E1BNK3 TGFB1 GO:0005515 GO_REF:0000024 ISS UniProtKB:P01137 F Transforming growth factor beta-1 TGFB1|TGFB1 protein taxon:9913 20141202 AgBase
100064 GO_AR:0000003 Warning Annotations to 'binding' (GO:0005488) and 'protein binding' (GO:0005515) should be made with an IPI evidence code and the interactor in the 'with' field UniProtKB P04272 ANXA2 GO:0005515 PMID:18597635 TAS F Annexin A2 ANXA2|ANXA2|ANX2 protein taxon:9913 20140514 AgBase
100960 GO_AR:0000003 Warning Annotations to 'binding' (GO:0005488) and 'protein binding' (GO:0005515) should be made with an IPI evidence code and the interactor in the 'with' field UniProtKB P08169 IGF2R GO:0005515 GO_REF:0000024 ISS UniProtKB:P11717 F Cation-independent mannose-6-phosphate receptor IGF2R|IGF2R|M6P protein taxon:9913 20091210 UniProt
102764 GO_AR:0000003 Warning Annotations to 'binding' (GO:0005488) and 'protein binding' (GO:0005515) should be made with an IPI evidence code and the interactor in the 'with' field UniProtKB P18341 TGFB1 GO:0005515 GO_REF:0000024 ISS UniProtKB:P01137 F Transforming growth factor beta-1 TGFB1|TGFB1 protein taxon:9913 20141202 AgBase
109065 GO_AR:0000003 Warning Annotations to 'binding' (GO:0005488) and 'protein binding' (GO:0005515) should be made with an IPI evidence code and the interactor in the 'with' field UniProtKB P80012 VWF GO:0005515 GO_REF:0000024 ISS UniProtKB:P04275 F von Willebrand factor VWF|VWF|F8VWF protein taxon:9913 20070328 UniProt
131516 GO_AR:0000003 Warning Annotations to 'binding' (GO:0005488) and 'protein binding' (GO:0005515) should be made with an IPI evidence code and the interactor in the 'with' field UniProtKB Q3MHX6 OS9 GO:0005515 GO_REF:0000024 ISS UniProtKB:Q13438 F Protein OS-9 OS9|OS9 protein taxon:9913 20090907 UniProt
131958 GO_AR:0000003 Warning Annotations to 'binding' (GO:0005488) and 'protein binding' (GO:0005515) should be made with an IPI evidence code and the interactor in the 'with' field UniProtKB Q3SX24 FBXO6 GO:0005515 GO_REF:0000024 ISS UniProtKB:Q9QZN4 F F-box only protein 6 FBXO6|FBXO6|FBS2|FBX6 protein taxon:9913 20090903 UniProt
#------------

Predictions

GO:0005623 cell AnnotationPropagation C GO:0035356 cellular triglyceride homeostasis ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005622 intracellular AnnotationPropagation C GO:0045047 protein targeting to ER ISS
GO:0005623 cell AnnotationPropagation C GO:0045047 protein targeting to ER ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005623 cell AnnotationPropagation C GO:0051560 mitochondrial calcium ion homeostasis ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0051560 mitochondrial calcium ion homeostasis ISS
GO:0005622 intracellular AnnotationPropagation C GO:0051168 nuclear export ISS
GO:0005623 cell AnnotationPropagation C GO:0051168 nuclear export ISS
GO:0005622 intracellular AnnotationPropagation C GO:0090110 cargo loading into COPII-coated vesicle ISS
GO:0005623 cell AnnotationPropagation C GO:0090110 cargo loading into COPII-coated vesicle ISS
GO:0016020 membrane AnnotationPropagation C GO:0090110 cargo loading into COPII-coated vesicle ISS
GO:0005622 intracellular AnnotationPropagation C GO:0072332 intrinsic apoptotic signaling pathway by p53 class mediator ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:1901029 negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway ISS
GO:0006508 proteolysis AnnotationPropagation P GO:0070006 metalloaminopeptidase activity ISS
GO:0005622 intracellular AnnotationPropagation C GO:1990126 retrograde transport, endosome to plasma membrane ISS
GO:0005623 cell AnnotationPropagation C GO:1990126 retrograde transport, endosome to plasma membrane ISS
GO:0016310 phosphorylation AnnotationPropagation P GO:0008887 glycerate kinase activity ISS
GO:0019918 peptidyl-arginine methylation, to symmetrical-dimethyl arginine AnnotationPropagation P GO:0035243 protein-arginine omega-N symmetric methyltransferase activity ISS
GO:0032259 methylation AnnotationPropagation P GO:0046539 histamine N-methyltransferase activity ISS
GO:0005622 intracellular AnnotationPropagation C GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress ISS
GO:0051726 regulation of cell cycle AnnotationPropagation P GO:0097472 cyclin-dependent protein kinase activity ISS
GO:0006468 protein phosphorylation AnnotationPropagation P GO:0097472 cyclin-dependent protein kinase activity ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0006898 receptor-mediated endocytosis AnnotationPropagation P GO:0070287 ferritin receptor activity ISS
GO:0005623 cell AnnotationPropagation C GO:0051281 positive regulation of release of sequestered calcium ion into cytosol ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005739 mitochondrion AnnotationPropagation C GO:0034553 mitochondrial respiratory chain complex II assembly ISS
GO:0005622 intracellular AnnotationPropagation C GO:0042073 intraciliary transport ISS
GO:0005623 cell AnnotationPropagation C GO:0042073 intraciliary transport ISS
GO:0005929 cilium AnnotationPropagation C GO:0042073 intraciliary transport ISS
GO:0005764 lysosome AnnotationPropagation C GO:1905146 lysosomal protein catabolic process ISS
GO:0005622 intracellular AnnotationPropagation C GO:0045039 protein import into mitochondrial inner membrane ISS
GO:0005623 cell AnnotationPropagation C GO:0045039 protein import into mitochondrial inner membrane ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0045039 protein import into mitochondrial inner membrane ISS
GO:0005622 intracellular AnnotationPropagation C GO:0072425 signal transduction involved in G2 DNA damage checkpoint ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0035435 phosphate ion transmembrane transport AnnotationPropagation P GO:0061513 glucose 6-phosphate:inorganic phosphate antiporter activity ISS
GO:0015712 hexose phosphate transport AnnotationPropagation P GO:0061513 glucose 6-phosphate:inorganic phosphate antiporter activity ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005739 mitochondrion AnnotationPropagation C GO:0070124 mitochondrial translational initiation ISS
GO:0005622 intracellular AnnotationPropagation C GO:1990126 retrograde transport, endosome to plasma membrane ISS
GO:0005623 cell AnnotationPropagation C GO:1990126 retrograde transport, endosome to plasma membrane ISS
GO:0005622 intracellular AnnotationPropagation C GO:0097193 intrinsic apoptotic signaling pathway ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0090615 mitochondrial mRNA processing ISS
GO:0006479 protein methylation AnnotationPropagation P GO:0071885 N-terminal protein N-methyltransferase activity ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0070933 histone H4 deacetylation AnnotationPropagation P GO:0097372 NAD-dependent histone deacetylase activity (H3-K18 specific) ISS
GO:0005622 intracellular AnnotationPropagation C GO:0072332 intrinsic apoptotic signaling pathway by p53 class mediator ISS
GO:0010952 positive regulation of peptidase activity AnnotationPropagation P GO:0016504 peptidase activator activity ISS
GO:0006508 proteolysis AnnotationPropagation P GO:0070573 metallodipeptidase activity ISS
GO:0005622 intracellular AnnotationPropagation C GO:0071880 adenylate cyclase-activating adrenergic receptor signaling pathway ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005622 intracellular AnnotationPropagation C GO:0061015 snRNA import into nucleus ISS
GO:0005623 cell AnnotationPropagation C GO:0061015 snRNA import into nucleus ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:1902445 regulation of mitochondrial membrane permeability involved in programmed necrotic cell death ISS
GO:0005622 intracellular AnnotationPropagation C GO:1903445 protein transport from ciliary membrane to plasma membrane ISS
GO:0005623 cell AnnotationPropagation C GO:1903445 protein transport from ciliary membrane to plasma membrane ISS
GO:0016020 membrane AnnotationPropagation C GO:1903445 protein transport from ciliary membrane to plasma membrane ISS
GO:0005622 intracellular AnnotationPropagation C GO:1990126 retrograde transport, endosome to plasma membrane ISS
GO:0005623 cell AnnotationPropagation C GO:1990126 retrograde transport, endosome to plasma membrane ISS
GO:0005622 intracellular AnnotationPropagation C GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0070125 mitochondrial translational elongation ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly ISS
GO:0016311 dephosphorylation AnnotationPropagation P GO:0098519 nucleotide phosphatase activity, acting on free nucleotides ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0070124 mitochondrial translational initiation IDA
GO:0030974 thiamine pyrophosphate transmembrane transport AnnotationPropagation P GO:0090422 thiamine pyrophosphate transmembrane transporter activity ISS
GO:0035461 vitamin transmembrane transport AnnotationPropagation P GO:0090422 thiamine pyrophosphate transmembrane transporter activity ISS
GO:0010950 positive regulation of endopeptidase activity AnnotationPropagation P GO:0061133 endopeptidase activator activity ISS
GO:0016311 dephosphorylation AnnotationPropagation P GO:0043874 acireductone synthase activity ISS
GO:0016310 phosphorylation AnnotationPropagation P GO:0051736 ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005739 mitochondrion AnnotationPropagation C GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic AnnotationPropagation P GO:0090624 endoribonuclease activity, cleaving miRNA-paired mRNA ISS
GO:0035279 mRNA cleavage involved in gene silencing by miRNA AnnotationPropagation P GO:0090624 endoribonuclease activity, cleaving miRNA-paired mRNA ISS
GO:0010452 histone H3-K36 methylation AnnotationPropagation P GO:0046975 histone methyltransferase activity (H3-K36 specific) ISS
GO:0120009 intermembrane lipid transfer AnnotationPropagation P GO:0120013 intermembrane lipid transfer activity ISS
GO:0043985 histone H4-R3 methylation AnnotationPropagation P GO:0044020 histone methyltransferase activity (H4-R3 specific) ISS
GO:0035246 peptidyl-arginine N-methylation AnnotationPropagation P GO:0044020 histone methyltransferase activity (H4-R3 specific) ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005622 intracellular AnnotationPropagation C GO:0090110 cargo loading into COPII-coated vesicle ISS
GO:0005623 cell AnnotationPropagation C GO:0090110 cargo loading into COPII-coated vesicle ISS
GO:0016020 membrane AnnotationPropagation C GO:0090110 cargo loading into COPII-coated vesicle ISS
GO:0006508 proteolysis AnnotationPropagation P GO:0036459 thiol-dependent ubiquitinyl hydrolase activity ISS
GO:0005622 intracellular AnnotationPropagation C GO:0090110 cargo loading into COPII-coated vesicle ISS
GO:0005623 cell AnnotationPropagation C GO:0090110 cargo loading into COPII-coated vesicle ISS
GO:0016020 membrane AnnotationPropagation C GO:0090110 cargo loading into COPII-coated vesicle ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:1901029 negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway ISS
GO:0005622 intracellular AnnotationPropagation C GO:0051168 nuclear export ISS
GO:0005623 cell AnnotationPropagation C GO:0051168 nuclear export ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0042407 cristae formation ISS
GO:0006508 proteolysis AnnotationPropagation P GO:0070006 metalloaminopeptidase activity ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005739 mitochondrion AnnotationPropagation C GO:0097345 mitochondrial outer membrane permeabilization ISS
GO:0098734 macromolecule depalmitoylation AnnotationPropagation P GO:0008474 palmitoyl-(protein) hydrolase activity ISS
GO:0005622 intracellular AnnotationPropagation C GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0097345 mitochondrial outer membrane permeabilization ISS
GO:0036065 fucosylation AnnotationPropagation P GO:0046920 alpha-(1->3)-fucosyltransferase activity ISS
GO:0005622 intracellular AnnotationPropagation C GO:0072425 signal transduction involved in G2 DNA damage checkpoint ISS
GO:0005622 intracellular AnnotationPropagation C GO:0097193 intrinsic apoptotic signaling pathway ISS
GO:0005623 cell AnnotationPropagation C GO:0099093 calcium export from the mitochondrion ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0033617 mitochondrial respiratory chain complex IV assembly ISS
GO:0035513 oxidative RNA demethylation AnnotationPropagation P GO:1990930 RNA N1-methyladenosine dioxygenase activity ISS
GO:0016311 dephosphorylation AnnotationPropagation P GO:0098519 nucleotide phosphatase activity, acting on free nucleotides ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005739 mitochondrion AnnotationPropagation C GO:0070126 mitochondrial translational termination ISS
GO:0005622 intracellular AnnotationPropagation C GO:0071880 adenylate cyclase-activating adrenergic receptor signaling pathway ISS
GO:0006412 translation AnnotationPropagation P GO:0008135 translation factor activity, RNA binding ISS
GO:0006468 protein phosphorylation AnnotationPropagation P GO:0098821 BMP receptor activity ISS
GO:0030509 BMP signaling pathway AnnotationPropagation P GO:0098821 BMP receptor activity ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:1902108 regulation of mitochondrial membrane permeability involved in apoptotic process ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0070145 mitochondrial asparaginyl-tRNA aminoacylation ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0006627 protein processing involved in protein targeting to mitochondrion ISS
GO:0005623 cell AnnotationPropagation C GO:0006878 cellular copper ion homeostasis ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0006470 protein dephosphorylation AnnotationPropagation P GO:0101006 protein histidine phosphatase activity IDA
GO:0005739 mitochondrion AnnotationPropagation C GO:0033617 mitochondrial respiratory chain complex IV assembly ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005739 mitochondrion AnnotationPropagation C GO:1990180 mitochondrial tRNA 3'-end processing ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005739 mitochondrion AnnotationPropagation C GO:0097345 mitochondrial outer membrane permeabilization ISS
GO:0007186 G-protein coupled receptor signaling pathway AnnotationPropagation P GO:0070915 lysophosphatidic acid receptor activity ISS
GO:0005623 cell AnnotationPropagation C GO:0051452 intracellular pH reduction ISS
GO:0005623 cell AnnotationPropagation C GO:0097277 cellular urea homeostasis ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0033617 mitochondrial respiratory chain complex IV assembly ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0016310 phosphorylation AnnotationPropagation P GO:0051731 polynucleotide 5'-hydroxyl-kinase activity ISS
GO:0016311 dephosphorylation AnnotationPropagation P GO:0052834 inositol monophosphate phosphatase activity IDA
GO:0006468 protein phosphorylation AnnotationPropagation P GO:0050254 rhodopsin kinase activity ISS
GO:0016311 dephosphorylation AnnotationPropagation P GO:0052629 phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005622 intracellular AnnotationPropagation C GO:1903445 protein transport from ciliary membrane to plasma membrane ISS
GO:0005623 cell AnnotationPropagation C GO:1903445 protein transport from ciliary membrane to plasma membrane ISS
GO:0016020 membrane AnnotationPropagation C GO:1903445 protein transport from ciliary membrane to plasma membrane ISS
GO:0055114 oxidation-reduction process AnnotationPropagation P GO:0070330 aromatase activity ISS
GO:0005622 intracellular AnnotationPropagation C GO:0072425 signal transduction involved in G2 DNA damage checkpoint ISS
GO:0005739 mitochondrion AnnotationPropagation C GO:0032543 mitochondrial translation HDA
GO:0005739 mitochondrion AnnotationPropagation C GO:0070901 mitochondrial tRNA methylation ISS
GO:0005622 intracellular AnnotationPropagation C GO:0072425 signal transduction involved in G2 DNA damage checkpoint ISS
GO:0071499 cellular response to laminar fluid shear stress FoldBasedPredictor GO:1903038 negative regulation of leukocyte cell-cell adhesion occurs_in CL:0002544 aortic endothelial cell part_of GO:0071499 cellular response to laminar fluid shear stress IMP
GO:0033267 axon part FoldBasedPredictor GO:0005874 microtubule part_of GO:0030426 growth cone part_of UBERON:0000955 brain IDA
GO:0030426 growth cone FoldBasedPredictor GO:0005874 microtubule part_of GO:0030426 growth cone part_of UBERON:0000955 brain IDA
GO:0120038 plasma membrane bounded cell projection part FoldBasedPredictor GO:0005874 microtubule part_of GO:0043005 neuron projection part_of UBERON:0000955 brain IDA
GO:0097458 neuron part FoldBasedPredictor GO:0005874 microtubule part_of GO:0043005 neuron projection part_of UBERON:0000955 brain IDA
GO:0043005 neuron projection FoldBasedPredictor GO:0005874 microtubule part_of GO:0043005 neuron projection part_of UBERON:0000955 brain IDA
GO:0042583 chromaffin granule FoldBasedPredictor GO:0097159 organic cyclic compound binding occurs_in GO:0042583 chromaffin granule IDA
GO:0033267 axon part FoldBasedPredictor GO:0098793 presynapse part_of GO:0030424 axon part_of UBERON:0002037 cerebellum IDA

Predictions (experimental)

N/A